研究目的
研究光激发GQD-Cur对牙周病原菌混合生物膜代谢活性的影响。
研究成果
光激发GQD-Cur对浮游态和生物膜态牙周病原菌均表现出强效抑制作用,并能下调生物膜相关基因表达谱,提示其作为治疗牙周病原菌混合生物膜的潜在优选方案。
研究不足
该研究仅在体外进行,效率有限。
1:实验设计与方法选择:
GQD-Cur的合成与表征、细胞毒性评估及其对牙周病原菌混合生物膜的影响研究
2:样本选择与数据来源:
包含伴放线聚集杆菌、牙龈卟啉单胞菌和中间普雷沃菌的牙周病原菌混合生物膜
3:实验设备与材料清单:
扫描电子显微镜(SEM)、动态光散射(DLS)、傅里叶变换红外光谱(FTIR)、紫外-可见分光光度计(UV-Vis)、X射线衍射仪(XRD)、蓝光LED
4:实验流程与操作步骤:
GQD-Cur对生物膜的光敏化处理、蓝光LED照射及细菌存活率、生物膜形成量、活性氧生成和基因表达的测定
5:数据分析方法:
采用单因素方差分析结合Tukey事后检验进行统计学分析
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X-ray diffraction
X’Pert PRO MPD
PANalytical Co.
Used to study the crystalline structure of GQD-Cur.
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NanoDrop spectrophotometer
ND-1000
Thermo Fisher Scientific
Used to ascertain the RNA concentration and quality.
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blue LED
DY400-4
Denjoy Dental Co., Ltd.
Used as a light source for photodynamic therapy.
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power meter
LaserPoint s.r.l
Used to measure the output powers during the experiment.
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scanning electron microscope
Used to determine the morphology and microstructure of GQD-Cur.
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dynamic light scattering
Malvern Zetasizer Nano ZS system
Used to measure the particle diameter of GQD-Cur.
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fourier transform infrared spectroscopy
Thermo Fisher Scientific
Used to analyze the chemical structure and bond formation of GQD-Cur.
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ultraviolet–visible spectrophotometer
Used to investigate the absorbance of the GQD-Cur solutions.
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microplate reader
Thermo Fisher Scientific
Used to measure the absorbance of the solution at 570 nm.
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fluorescence spectrophotometer
Used to measure the fluorescence intensity of DCF.
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Real-Time PCR Detection System
Line-GeneK
Bioer Technology
Used to carry out qRT-PCR reactions.
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