1：Experimental Design and Method Selection

The study involved the preparation of hydrophilic Cu-In-S quantum dots with aggregation-induced emission effect and their interaction with Zn2+ to observe the self-assembly into 3D networks.

2：Sample Selection and Data Sources

Cu-In-S quantum dots were prepared and their interaction with various cations, especially Zn2+, was studied to understand the aggregation-induced emission effect.

3：List of Experimental Equipment and Materials

UV-vis absorption and PL spectra were recorded by a Shimadzu UV-2100 photo spectrometer and a HORIBA FluoroMax-4 spectrofluorometer, respectively. Transmission electron microscopy (TEM) images were taken on a JEOL JEM-2100 microscope. The Field Emission Scanning Electron Microscope (FE-SEM) measurements were performed on a ZEISS Sigma 300.

4：Experimental Procedures and Operational Workflow

The quantum dots were prepared and their interaction with Zn2+ was studied by adding Zn2+ solution to the QDs solution and observing the changes in fluorescence. The morphology of the QDs in the presence or absence of Zn2+ was examined with TEM and SEM techniques.

5：Data Analysis Methods

The fluorescent spectra of mixed solution were recorded under the excitation of UV light at 380 nm. The factors affecting the efficiency of the proposed nanosensor, including the concentration of CIS QDs, the reaction time and the reaction temperature, were optimized.