研究目的
To enhance the sensitivity of turbidimetric inhibition immunoassay (TIIA) for biomarker detection by incorporating an optofluidic laser, enabling early diagnosis of diseases.
研究成果
OFL-TIIA significantly enhances the sensitivity of traditional TIIA, achieving a wide dynamic range and low limit of detection. This method provides a fast, sensitive, and low-cost immunoassay platform for disease diagnostics, with potential for widespread applications in early disease detection.
研究不足
The study focuses on enhancing the sensitivity of TIIA using an optofluidic laser, but the practical application may require further optimization for different biomarkers and clinical settings.
1：Experimental Design and Method Selection
The study proposes and demonstrates optofluidic laser TIIA (OFL-TIIA) for sensitive protein detection. The immunoreaction in OFL-TIIA takes place in a laser cavity, increasing the loss induced by antigen-antibody complexes (AACs) via the laser's amplification effect.
2：Sample Selection and Data Sources
A commercial IgG TIIA kit was used as a demonstrative model. The IgG standard solution was diluted to achieve a series of concentrations for the assay.
3：List of Experimental Equipment and Materials
Reflective mirrors (Thorlabs, USA), silica capillaries (Friedrich & Dimmock Inc.), 532 nm pulsed laser (CNI Laser Inc., China), portable spectrometer (Ocean Optics, USA), RhB (Aladdin Biotech Co., Ltd., China), and a commercial IgG TIIA kit (Erkn, China).
4：Experimental Procedures and Operational Workflow
The IgG standard solution was mixed with Tris-PEG buffer and incubated. The antibody solution was added and incubated to form AAC suspension. The mixture of AACs and RhB was injected into the capillary in the FP cavity, and the optofluidic laser emission was collected and analyzed.
5：Data Analysis Methods
The laser intensity was measured and analyzed to determine the IgG concentration, with the relationship between the spectrally integrated intensity and the IgG concentration plotted to establish the standard curve.
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