研究目的
Investigating the construction of bacterial biosensors using a supramolecular assembly for selective detection of E. coli.
研究成果
The study successfully demonstrated the development of a fluorescent supramolecular biosensor capable of selectively detecting E. coli. The biosensor's thermostability and the novel signal conversion mechanism based on conformational changes in the supramolecular assembly highlight its potential for durable and heatproof biosensor applications. Future research could expand the biosensor's applicability to other targets and environments.
研究不足
The study primarily focuses on E. coli detection, and the biosensor's performance with other bacterial strains or in complex environments was not extensively explored. The sensitivity and specificity under varying environmental conditions remain to be fully characterized.
1：Experimental Design and Method Selection:
The study utilized a double-layered octa-helical coiled-coil peptide assembly as the biosensor, incorporating tetravalent bioreceptors and environment-sensitive fluorophores. The design leveraged the noncovalent and reversible nature of the self-assembled system for signal conversion.
2：Sample Selection and Data Sources:
Escherichia coli (E. coli) and other bacteria (Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus cereus, Bacillus atrophaeus, and Bacillus thuringiensis) were used to test the biosensor's selectivity and sensitivity.
3：List of Experimental Equipment and Materials:
Peptides were synthesized using Fmoc solid phase synthesis techniques. Fluorescence and CD spectroscopy were used for characterization. Small-angle X-ray scattering (SAXS) and analytical ultracentrifugation (AUC) were employed for structural analysis.
4：Experimental Procedures and Operational Workflow:
The biosensor's interaction with bacteria was monitored through fluorescence emission changes. The thermostability of the biosensor was assessed via CD temperature ramp experiments.
5：Data Analysis Methods:
Fluorescence spectra were analyzed for intensity changes and Stokes shifts. SAXS data were processed to determine structural parameters.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
Confocal laser scanning microscope
LSM 710
Carl Zeiss
Used to obtain images of the SPN-bacteria interaction behavior.
ZEISS LSM 990 Spectral Multiplex
立即获取同款设备 -
Fluorescence spectrophotometer
LS-55
PerkinElmer
Used for steady-state fluorescence spectra collection.
暂无现货
预约到货通知
-
Peptide synthesizer
Tribute
Protein Technologies, Inc.
Used for peptide synthesis using standard Fmoc solid phase synthesis techniques.
暂无现货
预约到货通知
-
Circular Dichroism spectrometer
Chirascan
Applied Photophysics., Ltd.
Used for CD spectroscopy to monitor the self-assembling peptides.
暂无现货
预约到货通知
-
Analytical ultracentrifuge
ProteomeLab XL-A
Beckman Coulter, Inc.
Used for analytical ultracentrifugation experiments.
暂无现货
预约到货通知
-
登录查看剩余3件设备及参数对照表
查看全部
