研究目的
To develop a hand-held biosensing platform that integrates a plasmonic detection modality with a microfluidic chip for the detection of hemoglobin, aiming to improve point-of-care (POC) diagnostics.
研究成果
The hand-held plasmonic platform provides a versatile, portable, and easy-to-use approach for hemoglobin detection, with potential applications in POC settings. It offers label-free detection, simple user interface, and inexpensive disposable chips, addressing some of the key challenges in current biosensing technologies.
研究不足
The platform requires a pump for sample introduction, and non-specific binding in complex matrices like plasma can affect sensitivity and specificity. Further optimization with anti-fouling agents is needed for clinical applications.
1：Experimental Design and Method Selection:
The platform was designed based on Kretschmann configuration, integrating a plasmonic detection modality with a microfluidic chip. Theoretical simulations and kinetic calculations were performed to benchmark the platform performance.
2：Sample Selection and Data Sources:
Hemoglobin was used as a biological target model. Samples were prepared in PBS and clinically relevant media like plasma.
3：List of Experimental Equipment and Materials:
The platform consists of a 705 nm LED, a glass prism, a CMOS sensor, and a TV card for connection to a computer. Microfluidic chips were fabricated using PMMA, DSA, and gold-coated substrates.
4：Experimental Procedures and Operational Workflow:
Surface chemistry was applied to the plasmonic chip for hemoglobin detection. Real-time monitoring of resonance angle (R.A.) shifts was performed to assess binding events.
5：Data Analysis Methods:
Data was analyzed using theoretical simulations, equilibrium isotherm models (Scatchard, Langmuir, and Freundlich), and statistical analysis.
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