研究目的
Investigating the performance of a continuous-wave laser-induced forward transfer (CW-LIFT) system for printing liquid bioink and depositing cells in a controlled and precise manner.
研究成果
The CW-LIFT system demonstrated high printing quality and success rate, capable of depositing bioink and cells in a controlled and precise manner. Optimal printing conditions were identified, and the system's potential for biomedical applications was highlighted.
研究不足
The study is limited by the high cost of the laser system and the need for precise control over laser parameters to achieve high print quality. Additionally, scaling up the technique for fully three-dimensional structures remains a challenge.
1：Experimental Design and Method Selection:
The study utilized a CW-LIFT system incorporating an 830 nm infrared diode laser, laser-focusing optics, and a 16 nm gold thin film as the energy-absorbing layer.
2：Sample Selection and Data Sources:
The bioink used was Dulbecco’s modified Eagle medium (DMEM), and cell-laden droplets were prepared using MG63 and A549 cells.
3：List of Experimental Equipment and Materials:
Equipment included a motorized xy stage, a LabVIEW-based graphical user interface (GUI), and a sputter coater for gold film deposition.
4：Experimental Procedures and Operational Workflow:
The laser power, exposure time, and direct-writing height were varied to assess their effects on droplet formation and printing quality.
5：Data Analysis Methods:
The diameter of the laser influence zone and printed droplets were measured to evaluate printing performance.
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infrared diode laser
LD830-SE650
Thorlabs
Used as the energy source for the CW-LIFT system to induce the formation of tiny bioink droplets.
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motorized xy stage
PT1/M-Z8
Thorlabs
Used to control the 2D movement of the ribbon and receiving substrate for creating customized printed patterns.
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Alpha step pro?ler
Dektak 150
Veeco
Used to measure the thickness of the energy-absorbing layer by scanning the surface of the sample.
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microscope objective lens
CFI Super Plan Fluor ELWD 20×
Nikon
Used to highly focus the laser beam onto the energy-absorbing layer.
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sputter coater
108 Auto
TED PELLA
Used to coat the energy-absorbing layer (thin gold film) on the PMMA slide and silicon wafer.
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