研究目的
Investigating the influence of pulsed He–Ne laser irradiation on the interaction dynamics of red blood cells (RBCs) using optical tweezers to understand the underlying mechanisms of RBC aggregation and disaggregation, and the effects of laser irradiation on these processes.
研究成果
The study provided detailed insights into the dynamics of RBC interaction, showing that both the cell contact time and initial contact area influence the disaggregation force. Pulsed He–Ne laser irradiation at a specific frequency (225 Hz) was found to decrease RBC aggregation force after a short irradiation time (120 s), suggesting a potential method to modulate RBC aggregation properties. These findings contribute to the understanding of RBC interaction mechanisms and the effects of laser irradiation on blood properties.
研究不足
The study was conducted with RBCs from a single donor, which may limit the generalizability of the results. Additionally, the effects of laser irradiation were investigated over a short period (up to 300 s), and longer irradiation times may yield different outcomes. The mechanisms underlying the observed effects of pulsed laser irradiation on RBC aggregation require further investigation.
1：Experimental Design and Method Selection:
The study utilized a double-beam polarization-based optical tweezers system to investigate RBC interaction dynamics. The system was combined with a chopper-modulated pulsed laser irradiation module to study the effects of both continuous and pulsed low-level He–Ne laser irradiation on RBC aggregation.
2：Sample Selection and Data Sources:
Highly diluted RBCs suspensions in platelet-low autologous plasma from a single healthy donor were used. The samples were prepared and measured within 3 h at room temperature.
3：List of Experimental Equipment and Materials:
The setup included an infrared single-mode Nd:YAG laser source, a large numerical aperture water immersion objective, a He–Ne laser source, an optical chopper system, and a CMOS camera for observation.
4：Experimental Procedures and Operational Workflow:
RBC interaction was assessed by forming linear contact between two RBCs using OT, measuring aggregation and disaggregation forces, and applying laser irradiation to study its effects on RBC interaction.
5：Data Analysis Methods:
The interaction energy density between RBCs was calculated based on the measured forces and contact areas. Statistical analysis was performed to compare results under different conditions.
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