研究目的
To develop a simple, sensitive, and selective fluorometric method for the detection of folic acid (FA) using water-soluble AgInS2 quantum dots (QDs).
研究成果
Water-soluble AgInS2 QDs were successfully synthesized and demonstrated as effective fluorescent probes for the selective and sensitive detection of FA. The method offers a simple, rapid, and nontoxic approach for FA detection with potential applications in developing FA sensors.
研究不足
The study focuses on the detection of FA in aqueous solutions and may require further validation for complex biological samples. The synthesis and detection processes, while effective, could be optimized for higher sensitivity and selectivity.
1：Experimental Design and Method Selection:
The study utilized a fluorometric method for detecting FA based on the shift in the emission wavelength of AgInS2 QDs.
2：Sample Selection and Data Sources:
FA solutions of varying concentrations were prepared and added to AgInS2 QDs solutions for analysis.
3：List of Experimental Equipment and Materials:
Agilent Cary 50 ultraviolet–visible spectrophotometer, Shimadzu spectrophotometer, JEOL electron microscope (JEM-2100), D8 Focus diffractometer (Bruker).
4：Experimental Procedures and Operational Workflow:
Synthesis of AgInS2 QDs, characterization using various techniques, and detection of FA by observing changes in photoluminescence.
5：Data Analysis Methods:
The limit of detection (LOD) was calculated using the formula LOD = (3σ)/k, where σ is the standard deviation of the blank and k is the slope.
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