研究目的
To establish and validate in vivo confocal Raman spectroscopy for characterization of the lip barrier in conjunction with transepidermal water loss (TEWL) and skin capacitance assessments.
研究成果
In vivo confocal Raman spectroscopy provides insightful parameters for the characterization of the lip barrier and sufficiently low inter-individual variability to assess relatively small parameter changes employing relatively few study subjects. Differences at the molecular level and at a high spatial resolution are detectable, and these insights might provide a breakthrough in the evaluation of lip barrier function and developing solutions for lip care.
研究不足
The study was limited to healthy female volunteers aged 18-65 years with Fitzpatrick skin types I-III. The variability of NMF and ceramide content was high, which could affect the reproducibility of the results.
1：Experimental Design and Method Selection:
In vivo confocal Raman spectroscopy was employed to assess the lip barrier in conjunction with TEWL and skin capacitance assessments.
2：Sample Selection and Data Sources:
Thirty-two healthy volunteers were recruited, and a dental tongue fixation device was used to prevent lip moisturization during measurements.
3：List of Experimental Equipment and Materials:
Confocal Raman Spectrometer Model 3510 Skin Analyzer (River Diagnostics), Corneometer? (CM 825; Courage & Khazaka), Aquaflux? (AF200, Biox Systems Ltd.).
4：Experimental Procedures and Operational Workflow:
Raman spectra were obtained by focusing low power laser light in the skin and measuring the Raman scattered light. TEWL and skin hydration were measured using Aquaflux and Corneometer, respectively.
5：Data Analysis Methods:
Statistical analysis was performed to calculate means, standard deviations, and confidence intervals for all test parameters.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
