研究目的
To access protein molecular structure profile and metabolic characteristics of model forages in ruminant systems and quantify the relationship between forage protein molecular structures and protein utilization and availability.
研究成果
Non-invasive and non-destructive molecular spectroscopy (FT/IR-ATR) is capable to reveal the relationship between the protein molecular structures and protein rumen degradation and digestion characteristics of forage in dairy cows. The structure-nutrition interactive relationship study indicated that protein molecular structure profiles in forage were weakly associated to protein rumen degradation kinetics, but significantly correlated to protein subfractions, intestinal digestible protein, and truly absorbed nutrient supply to dairy cows.
研究不足
The study focused on model forages from specific sourced-origins (Foreign and Chinese), which may limit the generalizability of the findings to other types of forages or sourced-origins.
1：Experimental Design and Method Selection:
Advanced non-invasive vibrational molecular spectroscopic technique (ATR-FTIR) with chemometrics was applied to reveal forage protein molecular structure. Both univariate and multivariate molecular spectral analyses were applied to study molecular structure features in model forages.
2：Sample Selection and Data Sources:
Seven Foreign and five Chinese sourced-origin alfalfa hay (coded as: “FSO” n = 7 and “CSO” n = 5, respectively) as modeled forages were used for the molecular structure and conventional nutrition studies.
3：List of Experimental Equipment and Materials:
ATR-FTIR spectroscopy was used for molecular structure analysis.
4：Experimental Procedures and Operational Workflow:
The molecular structure study provided the detailed protein structure profiles of Amide I and Amide II areas and height, total Amide I and II area ratios, Amide I to II height ratio as well as Amide I to II area ratio using ATR-FTIR spectroscopy.
5：Data Analysis Methods:
Both univariate and multivariate molecular spectral analyses were applied to study molecular structure features in model forages.
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