研究目的
Developing a simple, efficient, and selective method for the qualitative and quantitative determination of oxytetracycline (OTC) in the environment.
研究成果
The study successfully developed a novel visual detection method for OTC using piperazine-modified CQDs, demonstrating a linear relationship between OTC concentration and fluorescence emission wavelength shift. This method offers a rapid, convenient, and selective approach for OTC detection in environmental samples.
研究不足
The method's effectiveness is pH-dependent, requiring alkaline conditions for optimal performance. The presence of other tetracyclines may interfere with the detection of OTC.
1:Experimental Design and Method Selection:
The study involved modifying carbon quantum dots (CQDs) with piperazine to create P-CQDs, which were then used to detect OTC through fluorescence resonance energy transfer (FRET).
2:Sample Selection and Data Sources:
OTC was used as the target analyte, with its concentrations varied to study the fluorescence response.
3:List of Experimental Equipment and Materials:
CQDs, piperazine, OTC, and various buffers for pH adjustment were used.
4:Experimental Procedures and Operational Workflow:
P-CQDs were synthesized and characterized, followed by fluorescence measurements in the presence of varying OTC concentrations.
5:Data Analysis Methods:
The relationship between OTC concentration and the fluorescence emission wavelength shift was analyzed.
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