研究目的
To address the severe issue of bacterial resistance by developing a photo-responsive norfloxacin antibiotic and constructing a self-assembled supramolecular photo-activated system to regulate the antibiotic activity.
研究成果
The study successfully developed a supramolecular photo-responsive antibiotic that exhibits a higher "on–off" ratio of antibacterial ability compared to the antibiotic alone. This approach combines supramolecular and light-regulating strategies to efficiently regulate antibiotic activity, offering potential to relieve the emergence of bacterial resistance.
研究不足
The study focuses on the in vitro antibacterial activity of the supramolecular photo-responsive antibiotic. The potential in vivo applications and long-term stability of the system were not explored.
1：Experimental Design and Method Selection:
The study involved the synthesis of a new photo-responsive antibiotic, azobenzene-norfloxacin (Azo-Nor), and its self-assembly with cyclodextrin (aCD) to form a supramolecular complex. The photoisomerization properties of Azo-Nor were studied via absorption spectra and 1H-NMR measurements.
2：Sample Selection and Data Sources:
The antibacterial activity of Azo-Nor and Azo-Nor/aCD was tested on Staphylococcus aureus subsp. (S. aureus) and Escherichia coli (E. coli).
3：List of Experimental Equipment and Materials:
Instruments used included absorption spectroscopy, 1H-NMR, isothermal titration calorimetry (ITC), dynamic light scattering (DLS), and scanning electron microscopy (SEM).
4：Experimental Procedures and Operational Workflow:
The study involved the synthesis of Azo-Nor, its characterization, the formation of the Azo-Nor/aCD complex, and the evaluation of its antibacterial activity before and after UV irradiation.
5：Data Analysis Methods:
The antibacterial activity was evaluated by determining the half maximal inhibitory concentration (IC50).
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