1：Experimental Design and Method Selection:

The study involved the synthesis of chiral gold nanopolyhedrons through peptide-directed growth, utilizing a specific peptide sequence (CGGEVSALEK) to functionalize gold nanoparticles. The methodology included the use of surfactants and reducing agents to control the morphology of the gold nanoparticles.

2：Sample Selection and Data Sources:

Gold nanoparticles with different morphologies (nanododecahedrons, nanotrioctahedrons, and nanocubes) were synthesized and functionalized with the peptide. The samples were characterized using various techniques to analyze their optical properties.

3：List of Experimental Equipment and Materials:

Materials included hexadecyltrimethylammonium bromide (CTAB), hexadecyltrimethylammonium chloride (CTAC), L-ascorbic acid (L-AA), sodium borohydride, silver nitrate, and tetrachloroauric trihydrate (HAuCl4·3H2O). Equipment used included a Jasco J-810 circular dichroism spectrometer, JEM-2100F transmission electron microscope, SIGMA system field emission scanning electron microscope (SEM), Nicolet 5 FT-IR spectrometer, UV-3600 UV-Vis spectrometer, and Nicomp 380 Z3000 particle size analyzer.

4：Experimental Procedures and Operational Workflow:

The synthesis involved the preparation of gold seed particles, growth solutions, and the functionalization of gold nanoparticles with the peptide. The nanoparticles were then characterized for their optical properties and morphology.

5：Data Analysis Methods:

The optical properties were analyzed using circular dichroism and UV-Vis spectroscopy. The morphology and size of the nanoparticles were analyzed using TEM, SEM, and dynamic light scattering.