研究目的
Investigating the use of two-dimensional (2D) ?uorescence spectroscopy as a monitoring tool for cultivation, harvesting, and e?uent treatment of Dunaliella salina with high carotenoid concentration to improve the production process and minimise costs.
研究成果
The study demonstrates the strong potential of combining 2D ?uorescence and chemometrics for monitoring different processes during microalgae production, including cell concentration, cell viability, and nitrate concentration. This approach can improve operational optimisation and control of Dunaliella salina cultivation, leading to better decision-making over the optimal point to start the induction of carotene production or to harvest.
研究不足
The study acknowledges the need for more experimental data at high cell concentration ranges to increase the robustness of the models. Additionally, the accuracy of cell viability models may be improved by incorporating physical parameters such as pH or salinity.
1：Experimental Design and Method Selection:
The study used 2D ?uorescence spectroscopy combined with chemometric analysis (PCA and PLS) to monitor cell concentration, cell viability, and nitrate concentration in media during the production of carotenoid-rich Dunaliella salina.
2：Sample Selection and Data Sources:
Samples were collected from cultivation batches, membrane harvesting experiments, and permeate treatment processes.
3：List of Experimental Equipment and Materials:
A ?uorescence spectrophotometer (Agilent Cary Eclipse, California, USA) equipped with monochromators for excitation and emission and an optical ?bre bundle probe was used.
4：Experimental Procedures and Operational Workflow:
Fluorescence spectra were acquired directly from algal suspensions without any further treatment.
5：Data Analysis Methods:
PCA was used to compress and deconvolute the excitation-emission matrices (EEMs) into principal components (PCs), and PLS modelling was used to describe multilinear correlations between the ?uorescence PCs and the offline measures of cell concentration, cell viability, and nitrate concentration.
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