研究目的
Investigating the use of a tapered singlemode-no core-singlemode (SNS) fiber structure as a biosensor for the detection of staphylococcus aureus, a foodborne pathogen.
研究成果
The tapered SNS fiber structure based biosensor demonstrated high sensitivity for staphylococcus aureus detection, with a wavelength shift of 1.1 nm for a concentration of 101 CFU/mL. This sensor structure can be adapted for detecting other foodborne pathogens by functionalizing different capture layers on the fiber sensor surface.
研究不足
The blue shift of the wavelength observed may be due to the thick coating of IgG on the fiber sensor surface, leading to weak binding between different layers of IgGs. Further experiments are needed to verify this.
1：Experimental Design and Method Selection:
A tapered SNS fiber structure was designed for refractive index sensing, with the NCF section tapered to increase evanescent field distribution and sensitivity.
2：Sample Selection and Data Sources:
Staphylococcus aureus solutions with concentrations of 101, 102, and 103 CFU/mL were used.
3：List of Experimental Equipment and Materials:
A commercial fiber tapering system (OC-2010, JILONG), broadband light source (BBS), optical spectrum analyzer (OSA), and various chemicals for sensor surface modification.
4：Experimental Procedures and Operational Workflow:
The NCF was tapered, the sensor surface was modified with pig IgG antibodies, and the sensor was tested with different concentrations of staphylococcus aureus.
5：Data Analysis Methods:
Spectral responses were measured to observe wavelength shifts indicative of staphylococcus aureus binding.
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