研究目的
To characterize the birefringence pattern of sperm heads using polarized digital holography microscopy as a label-free technique for assessing their healthy state and selecting reacted sperm cells.
研究成果
The polarized digital holographic microscope successfully characterized the birefringence pattern of sperm heads, demonstrating its potential as a label-free technique for assessing sperm health and selecting reacted sperm cells. This method could improve the success rate of intracytoplasmic sperm injection (ICSI) by providing a more accurate assessment of sperm quality.
研究不足
The study is a proof of concept, and further validation with a larger sample size and clinical trials is needed to confirm the applicability of the method in clinical settings.
1：Experimental Design and Method Selection:
The study utilized a polarized digital holographic microscope setup for transmission imaging, combining digital holography microscopy with polarization analysis to assess the birefringence properties of sperm cells.
2：Sample Selection and Data Sources:
Human sperm cells were used as samples to evaluate the amplitude, phase, and polarization state maps.
3：List of Experimental Equipment and Materials:
The setup included a He-Ne laser, beam splitters, polarizers, half and quarter wave plates, an objective lens, and a CCD for recording interference patterns.
4：Experimental Procedures and Operational Workflow:
The object beam was polarized and passed through the sample, with the transmitted wave collected by an objective lens. Two reference beams with orthogonal polarization were used to create interference patterns recorded by the CCD.
5：Data Analysis Methods:
The amplitude and phase maps of the two orthogonal components of the object field were reconstructed to evaluate the birefringence properties through the amplitude ratio β and phase difference Δ?.
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