研究目的
Investigating the applicability of hyperthermia to treating lysozyme amyloid fibrils (LAF)’s self-assembled fibrillary aggregates by a feedback-modulated temperature controller and near-infrared (NIR) laser-irradiated cesium tungstate (CsWO3) nanoparticles (NPs).
研究成果
The research demonstrated that thermal treatment and NIR laser-irradiated CsWO3 NPs can effectively disintegrate lysozyme amyloid fibrils. The findings suggest potential applications in developing therapeutic techniques for neurodegenerative diseases.
研究不足
The study focuses on in vitro conditions and may not fully replicate in vivo environments. The effects of hyperthermia and photothermal treatment on other types of amyloid fibrils were not explored.
1：Experimental Design and Method Selection:
The study employed atomic force microscopy (AFM), optical stereoscopy, and scanning electron microscopy (SEM) to investigate the interaction between LAF and NPs, and the effects of thermal treatment on fibrillary assemblies.
2：Sample Selection and Data Sources:
Lysozyme amyloid fibrils derived from hen egg white (HEWL) were cultured, and CsWO3 NPs were used for photothermal treatment.
3：List of Experimental Equipment and Materials:
Equipment included AFM (Veeco di Innova), SEM (SU-5000, Hitachi), UV-VIS-NIR optical absorbance spectroscopy (V-750, Jasco), and optical stereoscope (SMZ745T, Nikon). Materials included HEWL, glycine, HCl, NaCl, and CsWO3 NPs.
4：Experimental Procedures and Operational Workflow:
Samples were prepared by mixing LAF solution with NP solution, treated with thermal or NIR laser irradiation, and analyzed using AFM, SEM, and optical stereoscopy.
5：Data Analysis Methods:
Data were analyzed using Gwiddion analytic software for AFM images and other relevant software for SEM and optical data.
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