研究目的
To develop a novel and portable fluorescent sensor that integrates a lateral flow assay with a quantum dot (Qdots) label and a mobile phone reader for detection of specific antibodies in human serum, specifically for antibodies to the Taenia solium rT24H antigen.
研究成果
The developed POC test based on a combination of rT24H antigen and Qdots for antibody detection has similar performance to that of other assays for NCC. It demonstrates the feasibility for this test format and the potential use of mobile readers for qualitative reading of POC tests, opening the way for point of care diagnosis in cysticercosis control programs.
研究不足
The sensitivity of the assay was lower for the category of single viable cyst (44%) compared to two or more viable cysts (89%). The test may need further optimization for use as a screening tool for NCC.
1：Experimental Design and Method Selection:
The study was a retrospective analysis using serum samples from patients with neurocysticercosis (NCC) and controls. The assay integrated a lateral flow assay with a quantum dot label and a mobile phone reader.
2：Sample Selection and Data Sources:
Serum samples from 112 NCC patients and 152 controls were used. NCC diagnosis was confirmed by imaging.
3：List of Experimental Equipment and Materials:
Qdot 655 ITK carboxyl quantum dots, MES buffered saline, EDAC, Sulfo-NHS, PBS-BN, nitrocellulose membrane, and a mobile phone reader (Holomics Reader HRDR-30).
4：0).
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Serum samples were applied to the test strip, followed by a chase buffer. The test strip was then read using the mobile phone reader after 30 minutes.
5：Data Analysis Methods:
The ratio of the test line to the control line signals was calculated for each sample. ROC curves were created for different categories of NCC-positive samples.
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