研究目的
To design and develop novel fluorescent probes for the detection of biothiols in foods and human blood samples using maleimide modified graphene quantum dots and porphyrin fluorescence resonance energy transfer.
研究成果
The study successfully developed two fluorescent probes based on M-GQDs for biothiol detection. The FRET system demonstrated superior performance with a wider detection range and lower detection limit compared to the single M-GQDs system. The method was effectively applied to detect biothiols in foods and human blood samples, showing potential for practical applications in food safety and clinical diagnostics.
研究不足
The study did not explore the interference from other thiol-containing compounds in complex biological matrices. The practical application in diverse food matrices and clinical samples requires further validation.
1：Experimental Design and Method Selection:
The study involved the synthesis of maleimide functionalized GQDs (M-GQDs) and the development of a FRET system between M-GQDs and TAPP for biothiol detection. The Michael addition reaction was utilized for biothiol identification.
2：Sample Selection and Data Sources:
Biothiols (GSH and Cys) were used as test analytes. The detection was applied to fruit samples and human blood samples.
3：List of Experimental Equipment and Materials:
A Fluoromax-4NIR spectra fluorophotometer was used for fluorescence measurements. Materials included graphite powder, phosphorus pentoxide, tetrakis (4-aminophenyl) porphyrin, EDC, NHS, and others.
4：Experimental Procedures and Operational Workflow:
The synthesis of M-GQDs, the preparation of the FRET system, and the detection procedures for biothiols were detailed. The fluorescence spectra were measured under specific conditions.
5：Data Analysis Methods:
The fluorescence intensity ratio ITAPP/IGQDs was used for the detection of biothiols. Linear regression equations were established for quantification.
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