研究目的
Investigating the amplification of surface plasmon signals for a double hybridization microarray chip assembly to detect ultra-low concentrations of miRNA-155 marker in solution.
研究成果
The designed combined nano-bridge system has great potential for detection of small molecules at extremely low concentrations, with broader applicability for measuring most biological binding events feasible.
研究不足
The study is limited by the specific conditions required for the plasmonic signal amplification, including the precise matching of plasmonic wavelengths and the need for a fixed wavelength SPRi instrument.
1：Experimental Design and Method Selection:
The study employed a double hybridization microarray chip assembly bridging localized gold and detection probe-carrying-core/shell Fe3O4@Au nanoparticles.
2：Sample Selection and Data Sources:
miRNA-155 marker in solution was used as the analyte.
3：List of Experimental Equipment and Materials:
Core/shell Fe3O4@Au nanoparticles, localized gold nanoparticles, and a microarray chip were used.
4：Experimental Procedures and Operational Workflow:
The chip was fabricated with thiolated Au NPs through a linker of 1,4-benzenedithiol self-assembled monolayer (SAM), followed by chemisorption of the hairpin capture nucleotide probe onto the Au NPs.
5：Data Analysis Methods:
SPRi pixel intensities were measured for different concentrations of miRNA-155.
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