研究目的
The development of a rapid, sensitive, and selective fluorescence probe for the detection of ascorbic acid (AA) levels, which is significant for medical assay and diagnosis due to its association with many diseases.
研究成果
The ATP-Ce-Tris fluorescence probe demonstrated excellent sensitivity and selectivity for AA detection, with a wide linear range and low detection limit. Its applicability in human serum samples suggests potential for clinical diagnostic and drug screening applications.
研究不足
The study did not explore the long-term stability of the ATP-Ce-Tris nanoparticles under various environmental conditions or their potential toxicity in biological systems.
1：Experimental Design and Method Selection:
The study involved the synthesis of cerium coordination polymer nanoparticles (ATP-Ce-Tris) using ATP molecules as ligands to Ce3+ in Tris-HCl buffer. The fluorescence properties of ATP-Ce-Tris were investigated for AA detection.
2：Sample Selection and Data Sources:
Human serum samples were used to evaluate the practicality of the ATP-Ce-Tris fluorescence probe for AA detection.
3：List of Experimental Equipment and Materials:
Equipment included a Regulus 8010 scanning electron microscopy, TENSOR 27 FT-IR spectrometer, Hitachi U-3900 UV?Vis spectrophotometer, and F-4600 fluorescence spectrometer. Materials included adenosine triphosphate (ATP), cerium(III) nitrate hexahydrate, and various chemicals for buffer preparation.
4：Experimental Procedures and Operational Workflow:
The synthesis of ATP-Ce-Tris nanoparticles, optimization of sensing conditions, and AA detection in human serum samples were detailed step-by-step.
5：Data Analysis Methods:
Fluorescence spectra were analyzed to determine the sensitivity and selectivity of the ATP-Ce-Tris probe for AA detection.
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