研究目的
To develop an approach to the formation of robust collagen-based scaffolds using laser-induced curing of a photosensitive polylactide to improve the mechanical properties of collagen-based biomaterials.
研究成果
The developed technique of laser-induced curing allowed the creation of robust collagen-based scaffolds with significantly improved mechanical properties. The method also enabled the formation of fluorescent regions for potential noninvasive monitoring of biodegradation in vivo. The orientation patterns facilitated directed cell growth without compromising cytotoxicity.
研究不足
The study was limited by the potential for microcrack formation in the photocured regions due to thermomechanical stresses, especially at low laser speeds. The geometric configuration of the reinforcing pattern affected mechanical properties only at high laser radiation power densities.
1：Experimental Design and Method Selection:
The study involved the use of laser-induced curing of a photosensitive star-shaped polylactide to reinforce collagen films. The methodology included photochemical crosslinking of collagen in the presence of flavin mononucleotide (FMN) as a photoinitiator and directed laser-induced coating with reinforcing structures based on star-shaped polylactide.
2：Sample Selection and Data Sources:
Collagen was extracted from bovine dermis and processed into films. The films were then treated with FMN and subjected to UV irradiation for crosslinking.
3：List of Experimental Equipment and Materials:
Equipment included a LED source for UV irradiation, a laser stereolithography setup for laser patterning, and various microscopes and spectrometers for characterization. Materials included collagen, FMN, star-shaped polylactide, and dichloromethane for washing.
4：Experimental Procedures and Operational Workflow:
The collagen films were photocrosslinked with FMN, then coated with a photosensitive polylactide composition and subjected to laser patterning. The samples were washed to remove uncured polymer and characterized for mechanical properties, cytotoxicity, and other physico-chemical properties.
5：Data Analysis Methods:
Mechanical properties were analyzed using nanoindentation and stretching tests. Cytotoxicity was evaluated using MTT and LDH assays. IR spectroscopy and microscopy were used for chemical characterization.
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MDL-III-405 laser
MDL-III-405
CNI-Laser
Laser irradiation for patterning.
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FT-IR Spectrum Two spectrometer
Spectrum Two
PerkinElmer, Inc.
Acquiring IR spectra in the ATR mode.
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Spotlight 200i FT-IR Microscope
Spotlight 200i
PerkinElmer, Inc.
Performing reflection spectra of the patterns.
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Shimadzu EZTest EZ-SX universal testing machine
EZ-SX
Shimadzu
Performing stretching tests of collagen films.
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Multiscan FC microplate photometer
Multiscan FC
ThermoFisher Scientific
Quantifying absorbance at 550 nm for MTT-test.
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VICTOR Nivo Multimode Microplate Reader
VICTOR Nivo
Perkin Elmer
Measuring optical densities for LDH-test.
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Axiovert 200 fluorescence microscope
Axiovert 200
Karl Zeiss
Visualizing living and dead cells on reinforced films.
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Cary-50 spectrophotometer
Cary-50
Varian Optical Spectroscopy Instruments
Measuring the optical density at the wavelength of 444 nm for FMN absorption band analysis.
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LED source
Epileds
Epileds
UV irradiation for photocrosslinking of collagen films.
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Piuma Nanoindenter
Piuma
Optics11
Measuring local mechanical characteristics of collagen films.
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KOZO XJF900 fluorescent microscope
XJF900
KOZO OPTICS
Visualizing created patterns after 3D printing.
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UCMOS14000KPA digital camera
UCMOS14000KPA
ToupCam
Digital imaging for fluorescent microscope.
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Phenom Pro X SEM
Phenom Pro X
Phenom-World BV.
Estimating topography of individual created lines.
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Huvitz HRM 3D microscope
HRM
Huvitz
Evaluating width and 3D relief of formed lines.
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