摘要： Simultaneous detections of di?erent but clinically relevant biomarkers are of extremely importance in biomedicine. Due to their unique photophysical properties, quantum dots (QDs) are ideally suited for highly sensitive multiplexed determination. Herein, a dual quantum dots-based ?uorescence-linked immunosorbent assay (dQDs-FLISA) for simultaneous and quantitative detection of in?ammation biomarkers (i.e. serum amyloid A (SAA) and C-reactive protein (CRP)) has been established. After being coated by amphiphilic oligomers (poly-maleic acid n-hexadecanol ester, PMAH), the red-QD and green-QD with high quantum yields were used as ?uorescence probes to couple SAA and CRP antibodies, respectively. Cross-reactivity among the SAA and CRP’s antibodies and antigens have been carefully examined by interference experiments, and it was successfully avoided by the speci?c probe adding sequence. Therefore, the assay provided a broad linear analytical range, including SAA quantitative range of 10–1,000 ng mL?1 with linear correlation (R2) of 0.992, and CRP quantitative range of 10–1,000 ng mL?1 with R2 of 0.998. The limit of detections (LODs) for SAA and CRP using dQDs-FLISA were 2.39 ng mL?1 and 6.37 ng mL?1, respectively. The accuracy of the assay has been con?rmed with recoveries of 92.13%–101.85%. More importantly, the assay results showed good speci?city, the QD-antibody probe could couple corresponding antigen (CRP or SAA) high-e?ciently and it could be out of interference of other antigens and substances in serum. Given its good performance, the proposed dQDs-FLISA method o?ers great potential for simultaneous and quantitative detection of other biomarkers in in vitro diagnostic (IVD).