研究目的
To evaluate the anti-inflammatory effect of photodynamic therapy using guaiazulene and a red laser on peripheral blood mononuclear cells (PBMCs).
研究成果
Photodynamic therapy using guaiazulene and 635 nm red laser had an anti-inflammatory effect in peripheral blood mononuclear cells and fibroblast cells without endangering the cells. ESR and fluorescence probes confirmed that 2-5 μM guaiazulene irradiated with 4-8 J/cm2 generated the greatest amount of singlet oxygen and decreased RANTES and PGE2 the most when using the ELISA test.
研究不足
The study used a single cell layer model which may behave differently from stratified layers of a cell in vivo. The incubation time of 30 minutes may not be the optimum time for cell permeation.
1：Experimental Design and Method Selection:
The study involved irradiating guaiazulene solutions with red laser light to measure ROS generation using ESR spectroscopy and fluorescence.
2：Sample Selection and Data Sources:
PBMCs were used as the tissue model for inflammatory disease.
3：List of Experimental Equipment and Materials:
Guaiazulene solutions, red laser light, black 96-well plates, ESR spectrometer, fluorescence probes (DMA and APF), ELISA kits for RANTES and PGE
4：Experimental Procedures and Operational Workflow:
Guaiazulene solutions were irradiated with red laser light, and ROS were measured. Cell viability and anti-inflammatory effects were assessed.
5：Data Analysis Methods:
Statistical analyses were performed using Kruskal-Wallis and Dunn Bonferroni tests.
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