研究目的
To investigate the mechanism of LED treatment in maintaining the quality of pakchoi by integrating transcriptomic and metabolomic data.
研究成果
LED irradiation delays the senescence of postharvest pak-choi by activating photosynthesis, inducing glucosinolate biosynthesis, and inhibiting the down-regulation of porphyrin and chlorophyll metabolism pathways. This approach can extend the shelf life and improve the postharvest quality of pak-choi.
研究不足
The study focuses on the effects of white LED light on pak-choi, and the findings may not be directly applicable to other types of light or vegetables. The study also relies on specific storage conditions (20 °C, 90% relative humidity) which may limit the generalizability of the results.
1：Experimental Design and Method Selection
The study used low-intensity white LED light to treat postharvest pakchoi and analyzed the effects using transcriptomic and metabolomic profiling.
2：Sample Selection and Data Sources
Pak-choi plants were harvested at their optimum harvest date, selected for quality, and stored under dark or LED light conditions. Samples were collected at specific intervals for analysis.
3：List of Experimental Equipment and Materials
LED light at wavelengths of 448 nm and 549 nm, Lighting Passport (ALP-01, Asensetek, Taiwan), NanoDrop 2000, Agilent Bioanalyzer 2100 system, NEBNext UltraTM RNA Library Prep Kit for Illumina, ELISA Kit (LMAI Bio, Shanghai, China), microplate reader (Multiskan GO, Thermo Scientific).
4：Experimental Procedures and Operational Workflow
Pak-choi was stored under continuous white LED light or dark conditions. RNA-seq library preparation and sequencing were performed. Metabolomic analysis was conducted alongside transcriptomic analysis.
5：Data Analysis Methods
Differential expression analysis was performed using DESeq R package. KEGG pathway enrichment analysis was used to identify affected pathways. Pearson correlation coefficients and canonical correlation analysis were used for correlation analysis.
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microplate reader
Multiskan GO
Thermo Scientific
Used to measure absorbance at 450 nm for chlorophyllide a content determination.
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LED light
448 nm and 549 nm
Used to irradiate pak-choi to delay senescence and maintain quality during storage.
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Lighting Passport
ALP-01
Asensetek
Used to measure light intensity.
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NanoDrop 2000
Thermo Fisher Scientific
Used to measure RNA concentration.
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Agilent Bioanalyzer 2100 system
Agilent Technologies
Used to assess RNA integrity.
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NEBNext UltraTM RNA Library Prep Kit for Illumina
NEB
Used for RNA sample preparations.
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ELISA Kit
LMAI Bio
Used to determine chlorophyllide a content.
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