研究目的
To discriminate the vertical spacing of ?uorescent molecules in a guided mode structure by constructing a function that depends only on depth and wavelength.
研究成果
The study demonstrates a method to obtain the vertical spacing of ?uorescent molecules in a waveguide structure by constructing a criterion function based on the intensity ratio between waveguide modes. This method provides an alternative potential use for leakage radiation microscopy (LRM) and can be used to improve imaging and sensor researches with ?uorescent probes in vertical coupled structures.
研究不足
The method requires the structural parameters of the waveguide structure to be known and is limited by the numerical aperture of commonly used oil immersion objectives.
1：Experimental Design and Method Selection:
Utilizes a leakage radiation microscope (LRM) to collect directional ?uorescence radiation information from the back focal plane. The method involves analyzing the intensity ratio between waveguide modes to determine the vertical distance between ?uorescent molecules.
2：Sample Selection and Data Sources:
Fluorescent dimer with different gaps embedded into polymer layers on a waveguide structure consisting of a glass substrate, metallic silver ?lm, and PMMA polymer layer.
3：List of Experimental Equipment and Materials:
Leakage radiation microscope, waveguide structures (glass substrates, metallic silver ?lms, PMMA polymer layers), ?uorescent molecules.
4：Experimental Procedures and Operational Workflow:
Excitation of ?uorescent molecules, collection of ?uorescence radiation information from the back focal plane, analysis of intensity ratio between waveguide modes.
5：Data Analysis Methods:
Construction of a criterion function based on the intensity ratio between different waveguide modes to determine the vertical spacing of ?uorescent molecules.
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