研究目的
Investigating the synthesis of yellow emissive nitrogen-doped graphene quantum dots (N-GQDs) for Fe3+ sensing and bioimaging applications.
研究成果
The synthesized N-GQDs exhibit high fluorescence quantum yield, favorable biocompatibility, and strong resistance to photobleaching, making them suitable for Fe3+ detection in natural water and cellular imaging. The detection limit of 63 nM demonstrates high sensitivity and selectivity for Fe3+.
研究不足
The study focuses on Fe3+ detection and bioimaging, potentially limiting its applicability to other metal ions or broader sensing applications.
1:Experimental Design and Method Selection:
Hydrothermal synthesis of N-GQDs using dopamine as a precursor.
2:Sample Selection and Data Sources:
Dopamine hydrochloride and other chemicals were purchased from Aladdin.
3:List of Experimental Equipment and Materials:
TEM, AFM, XRD, XPS, TGA, UV-vis spectrophotometer, luminescence spectrometer, TCSPC technique, Raman scattering, FT-IR spectrograph.
4:Experimental Procedures and Operational Workflow:
Synthesis of N-GQDs, characterization, quantum yield measurements, detection of Fe3+, in vitro fluorescence imaging of cells.
5:Data Analysis Methods:
PL spectra analysis, Stern-Volmer equation for quenching mechanism, linear relationship between fluorescence and Fe3+ concentration.
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Transmission Electron Microscopy
Hitachi H-8100
Hitachi
Characterization of N-GQDs
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X-ray diffraction
D/Max
Rigaku
Determination of the structure of N-GQDs
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Luminescence spectrometer
PerkinElmer LS55
PerkinElmer Instruments
Collection of PL and PLE spectra
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Time-correlated single-photon counting
Hydra Harp 400
Pico Quant
Measurement of PL lifetime
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Atomic Force Microscope
Cypher S
Asylum Research
Determination of the height profiles of N-GQDs
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X-ray photoelectron spectroscopy
PHI Quantera II system
Ulvac-PHI INC.
Determination of the surface chemical composition and bonding states of N-GQDs
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Thermogravimetry
Pyres 1 TGA
Investigation of the thermal decomposition and thermal stabilities of N-GQDs
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Ultraviolet-visible spectrophotometer
UV-5800
Characterization of UV-vis absorption properties
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Raman scattering
SENTERRA
Determination of the quality
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Fourier transform infrared spectroscopy
Nicolet 670
Measurement of FT-IR spectrum
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