研究目的
To develop stable and biocompatible NIR-II fluorophores suitable for in vivo applications, specifically focusing on the synthesis of PEGylated dendrimer-encapsulated Ag2S QDs for NIR-II biological imaging.
研究成果
The one-pot synthesis of PEG-PATU Ag2S QDs provides a facile method for creating stable and biocompatible NIR-II fluorophores. These QDs exhibit strong fluorescence, good stability, and are suitable for in vivo imaging, including tracking cancer cell mobility and visualizing the vascular system. This method opens new perspectives for the preparation of novel NIR-II fluorescence nanoprobes for biolabeling and targeted imaging.
研究不足
The study focuses on the synthesis and preliminary in vivo application of PEG-PATU Ag2S QDs. Further optimization may be required for broader biomedical applications, including targeting specificity and long-term biocompatibility studies.
1：Experimental Design and Method Selection:
A one-pot strategy was used to synthesize water-dispersible, PEGylated dendrimer encapsulated Ag2S QDs clusters. Silver ions were loaded into the core of acylthiourea-functionalized dendrimer (PEG-PATU) through coordination, followed by the addition of sodium sulfide to form Ag2S QDs in situ.
2：Sample Selection and Data Sources:
Human lung adenocarcinoma (A549) cancer cells were used for cellular uptake and in vivo tracking experiments.
3：List of Experimental Equipment and Materials:
Transmission electron microscopy (TEM), high-resolution TEM (HR-TEM), dynamic light scattering (DLS), UV-Vis-NIR spectrophotometer, NIR fluorescence emission spectrometer, energy dispersive spectrum (EDS) images, inductively coupled plasma mass spectrometry (ICP-MS).
4：Experimental Procedures and Operational Workflow:
PEG-PATU was synthesized and used as a template to in situ generate Ag2S QDs. The size, absorbance, and fluorescence emission spectra of the QDs were measured at different reaction times. Cellular uptake and in vivo imaging were performed.
5：Data Analysis Methods:
The fluorescence quantum yields of PEG-PATU Ag2S QDs were calculated using a standard sample (IR-26). Cellular uptake was quantified using ICP-MS.
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