研究目的
To develop a graphene-based approach for the direct, high-throughput delivery of fluorescent probes into adherent cells to enable in situ live-cell super-resolution microscopy (SRM) on the same device within minutes.
研究成果
The graphene-based electroporation method enables efficient delivery of a wide range of fluorescent probes into adherent cells, facilitating high-quality super-resolution microscopy. The method's spatial and temporal controls allow for patterned delivery of different probes, offering a versatile platform for live-cell imaging and potential therapeutic applications.
研究不足
The approach requires precise control over electroporation parameters and graphene electrode integrity. Potential limitations include variability in delivery efficiency across different cell types and probe sizes.
1：Experimental Design and Method Selection:
Utilized graphene's properties for electroporation-based probe delivery into adhered mammalian cell lines and primary cells, followed by in situ SRM imaging.
2：Sample Selection and Data Sources:
Adherent mammalian cells, including cell lines (A549 and PtK2 cells) and primary cells (rat hippocampal neurons and neural stem cells), cultured on graphene surface.
3：List of Experimental Equipment and Materials:
Graphene electrodes, electroporation buffer, fluorescent probes (e.g., SR101, phalloidin-AF488, AF647-conjugated IgG), commercial electroporator, inverted fluorescence microscope.
4：Experimental Procedures and Operational Workflow:
Cells cultured on graphene, electroporation performed with voltage pulse, incubation with probes, washout, and SRM imaging.
5：Data Analysis Methods:
Fluorescence microscopy and STORM SRM for high-resolution imaging.
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