研究目的
Developing a selective and sensitive fluorescence probe for the detection of cysteine (Cys) with colorimetric and fluorescence dual signal changes, capable of discriminating Cys from other biothiols like homocysteine (Hcy) and glutathione (GSH).
研究成果
The developed fluorescent probe offers a selective and sensitive method for detecting Cys with colorimetric and fluorescence dual signal changes, capable of discriminating Cys from Hcy and GSH. It has potential applications in bioimaging of endogenous Cys in living cells, with minimal cytotoxicity.
研究不足
The probe's sensitivity is optimal at pH over 8, which may limit its application in more acidic environments. Additionally, the study focuses on Cys detection, with less emphasis on other biothiols under varying physiological conditions.
1：Experimental Design and Method Selection:
The probe was synthesized via sulfonamide reaction coupling between a sulfonyl benzoxadiazole (SBD) dye and dansyl chloride linked with a rigid piperazine group. The chloride unit at the 4-position on the SBD dye serves as the Cys-specific recognition site.
2：Sample Selection and Data Sources:
The response of the probe to Cys/Hcy/GSH was investigated using UV and FL spectroscopic techniques in aqueous solution.
3：List of Experimental Equipment and Materials:
A Cary 60 UV visible spectrophotometer and a Varian Cary Eclipse Fluorescence spectrophotometer were used for measurements.
4：Experimental Procedures and Operational Workflow:
The probe's response to Cys was tested over a pH range from 2 to 12, and its practicality for cell imaging was explored in living MCF-7 cells.
5：Data Analysis Methods:
The fluorescence intensity changes were analyzed to determine the probe's sensitivity and selectivity towards Cys.
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