研究目的
Evaluating the effect of different LED wavelengths and photoperiods on protein productivities and free amino acid (FAA) profile of Spirulina sp. LEB 18 cultures.
研究成果
LEDs, particularly red and green wavelengths, significantly increase protein productivity and FAA concentration in Spirulina sp. LEB 18 cultures, offering a sustainable alternative to traditional light sources.
研究不足
The study focused on Spirulina sp. LEB 18 and may not be directly applicable to other microalgae species. The light intensities and wavelengths used are specific to the experimental setup.
1：Experimental Design and Method Selection:
Different wavelengths of LEDs (blue, green, red, and white) and photoperiods (partial and integral) were evaluated in Spirulina sp. LEB 18 cultures. Control culture was performed using fluorescent light.
2：Sample Selection and Data Sources:
Spirulina sp. LEB 18 from the Culture Collection of the Laboratory of Biochemical Engineering at Federal University of Rio Grande (FURG) was used.
3：List of Experimental Equipment and Materials:
LEDs ribbons, fluorescent lamps, photobioreactors, light meter (WALZ ULM-500), centrifuge (Hitachi Himac CR-GIII), lyophilizer (Labconco), gas chromatograph (Varian 3400).
4：0). Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: Cultures were maintained at 30 °C for 10 days, with agitation provided by compressed air. Biomass was recovered by centrifugation, lyophilized, and stored for analysis.
5：Data Analysis Methods:
Protein content was determined via Lowry method, and FAA were analyzed by GC-FID. Statistical analysis was performed using ANOVA followed by Tukey’s test.
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