研究目的
Investigating the high-sensitivity biosensor for identification of protein based on terahertz Fano resonance metasurfaces.
研究成果
The terahertz ASRs biosensors fabricated on flexible polyimide substrate demonstrated high sensitivity detection for protein A/G and IgG, with refractive index sensitivities of 160 GHz/RIU and 240 GHz/RIU at two transmission dips. The results illustrated the ASRs biosensors' high sensitivity and label-free detection for proteins, providing a new feasible way of sensing in terahertz domain and potential application on protein binding process.
研究不足
The study does not discuss the potential for optimization of the biosensor's selectivity, sensitivity, and applicability for biosensings, nor does it address the scalability of the fabrication process.
1：Experimental Design and Method Selection:
The study proposed a planar array of Fano asymmetric split ring resonators (ASRs) fabricated on 25 μm flexible polyimide substrate for terahertz metasurface biosensors. The design aimed to achieve high sensitivity and label-free detection of proteins.
2：Sample Selection and Data Sources:
The biosensor samples included protein A/G and A/G + IgG spotted on ASRs surface. The samples were prepared by spotting 1 mg/mL protein A/G on ASRs surface, followed by spotting IgG with 1 mg/mL on protein A/G.
3：List of Experimental Equipment and Materials:
The ASRs arrays were fabricated on flexible PI film by conventional UV lithography. Materials included polyimide (PI) substrate, copper (Cu), positive photoresist (SUN-115P), and developer (SUN-238D). Equipment included THz time domain spectroscopy (THz-TDs) system (Batop, TDS1008) for experimental transmission measurements.
4：Experimental Procedures and Operational Workflow:
The fabrication process involved fixing a PI film on a silicon slab, depositing Cu, coating with photoresist, UV lithography, etching, and removing photoresist residue. The biosensor samples were prepared by spotting proteins on ASRs surface and incubating.
5：Data Analysis Methods:
The sensitivity of the biosensor was characterized by the shift in resonance frequency (Δf) relative to the change in refractive index (Δn), defined as S = Δf /Δn. The quality factor (Q) and figure of merit (FOM) were also calculated to describe the sensing performance.
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