研究目的
To develop fluorescence imaging acquisitions with improved dynamic range, focusing on the development of a new technical approach for high dynamic range confocal and two-photon microscopy that extends the imaging dynamic range in fluorescence optical scanning microscopy.
研究成果
The development of high dynamic range fluorescence imaging techniques has been demonstrated to improve the visualization of both dim and bright structures within biological samples, enabling accurate measurements of fluorophore concentration and better quantification via improved segmentation of cellular and dendritic structures. These technologies are expected to gain importance in capturing cellular distribution information at the whole organ level and in vivo studies for quantification of drug pharmacokinetics.
研究不足
The limited dynamic range of current photodetectors and imaging CCDs, and the challenge of accurately segmenting and quantifying cellular and dendritic structures in biological samples with varying fluorescence intensities.
