研究目的
To develop and evaluate biomimetic 3D printed nerve guidance channels (NGCs) filled with aligned cryomatrix and nerve growth factor (NGF) for enhanced peripheral nerve regeneration.
研究成果
The 3D printed aligned cryomatrix filled NGCs with NGF demonstrated enhanced peripheral nerve regeneration, comparable to autografts, by providing topographical and biochemical cues. This approach offers a promising alternative for nerve repair, especially for critical size defects.
研究不足
The study was limited to a rat model, and the long-term effects and scalability for human applications were not explored. The degradation rate of the cryogel and its impact on nerve regeneration over longer periods were not fully characterized.
1：Experimental Design and Method Selection:
The study involved the synthesis of photocrosslinkable polycaprolactone (PCL) for 3D printing of NGCs using stereolithography. Aligned and random cryogels were fabricated within the NGCs to provide topographical cues. NGF was incorporated to provide biochemical cues.
2：Sample Selection and Data Sources:
Rat sciatic nerve injury model was used for in vivo evaluation.
3：List of Experimental Equipment and Materials:
Included 3D printing setup, SEM for morphological analysis, rheometer for viscosity measurement, and ELISA for NGF release kinetics.
4：Experimental Procedures and Operational Workflow:
NGCs were implanted in rats, and functional recovery was assessed through electrophysiology and walking track analysis. Histological and immunohistochemical analyses were performed post-sacrifice.
5：Data Analysis Methods:
Statistical analysis was performed using GraphPad Prism for comparing groups.
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