研究目的
Investigating the potential of luminescent silica mesoparticles (SMPs) doped with octahedral molybdenum clusters for cellular labeling and protein transduction into larynx carcinoma (Hep-2) cells using GFP as a model protein.
研究成果
Luminescent SMPs doped with molybdenum clusters are highly attractive for biomedical applications, including bioimaging, biolabeling, and cellular delivery of biological molecules like proteins. They are non-toxic, efficiently internalize into cells, and can be functionalized to deliver cargo, offering a cheap and trackable alternative to existing materials.
研究不足
The study is limited to in vitro applications using a specific cell line (Hep-2). Further in vitro and in vivo studies are needed to fully exploit the potential of SMPs for biomedical applications.
1：Experimental Design and Method Selection:
The study involved the synthesis of luminescent SMPs doped with molybdenum clusters via a one-pot reaction, functionalization of SMPs with glycidyl groups, and conjugation with GFP.
2：Sample Selection and Data Sources:
Human larynx carcinoma cell line (Hep-2) was used for cellular uptake and cytotoxicity studies.
3：List of Experimental Equipment and Materials:
TEM (Libra 120, Zeiss), CytoFlexS (Beckman Coulter, USA), IN Cell Analyzer (GE Healthcare, USA), and various chemicals from Alfa Aeser, Sigma Aldrich, or Fluka.
4：Experimental Procedures and Operational Workflow:
SMPs were synthesized, functionalized, and conjugated with GFP. Cellular uptake, cytotoxicity, and photo-induced cytotoxicity were assessed using fluorescent microscopy, flow cytometry, and MTT-assay.
5：Data Analysis Methods:
Statistical analysis was performed using the Mann-Whitney U test, with data presented as mean values ± SEM.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
