研究目的
To investigate the feasibility of using platinum complex for potential applications in diagnostics, specifically as a novel photoacoustic contrast agent.
研究成果
The study successfully demonstrated a biodegradable PA contrast agent combining platinum complex and biodegradable polymer, offering a versatile approach for brain vascular imaging. The polymer's properties, such as longevity and intracellular penetration, make it a useful platform for developing PA contrast agents for preclinical and clinical applications.
研究不足
The study is limited by the complexity of transition metal complexes and potential toxicity issues, although the reported polymer shows low cytotoxicity. The PA imaging technique's penetration depth is also a limitation.
1：Experimental Design and Method Selection:
The study involved the synthesis of a platinum II-based biodegradable polymer for photoacoustic imaging, characterization of the polymer, and evaluation of its performance as a contrast agent in vitro and in vivo.
2：Sample Selection and Data Sources:
MDA-MB-231 cell lines and male Wistar rats were used for in vitro and in vivo studies, respectively.
3：List of Experimental Equipment and Materials:
Instruments included a Bruker DRX-400 NMR spectrometer, Waters 2690 apparatus for GPC, Philips CM300 FEGTEM for TEM, and a custom-designed PAM system. Materials included 4-aminomethyl piperidine, N,N`-bis(acryloyl) cystamine, cisplatin, and others.
4：Experimental Procedures and Operational Workflow:
The synthesis of the polymer, preparation of cisplatin incorporated micelles, cytotoxicity assay, fluorescence labeling, and PA imaging were performed.
5：Data Analysis Methods:
Data were analyzed using ICP-MS for platinum content, DLS for particle size, and PA signal analysis for imaging.
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Bruker DRX-400 NMR spectrometer
DRX-400
Bruker
Recording 1H NMR spectra
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DET36A/M
DET36A/M
Thorlabs
Monitoring fluctuations in the laser energy
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Waters 2690 apparatus
2690
Waters
Gel permeation chromatography (GPC)
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Philips CM300 FEGTEM
CM300
Philips
Transmission electron microscopy (TEM)
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Brookhaven BI-9000AT Digital Autocorrelator
BI-9000AT
Brookhaven
Dynamic light scattering (DLS) measurements
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Waters 2695 Separation Module
2695
Waters
Isocratic reverse phase high performance liquid chromatography (HPLC)
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Surlite OPO Plus
OPO Plus
Continuum
Generating laser pulses for photoacoustic microscopy
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Surlite II-10
II-10
Continuum
Pumping the optical parametric oscillator
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50-MHz ultrasonic transducer
Acoustic Sensor Co., Ltd
Receiving PA signals in the PAM system
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CompuScope 14220
14220
GaGe
Digitizing and sampling PA signals
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