摘要： Proper cellular proteostasis is essential to cellular ?tness and viability. Exogenous stress conditions compromise proteostasis and cause aggregation of cellular proteins. We have developed a ?uorogenic sensor (AgHalo) to quantify stress-induced proteostasis de?ciency. The AgHalo sensor uses a destabilized HaloTag variant to represent aggregation-prone cellular proteins and is equipped with a series of ?uorogenic probes that exhibit a ?uorescence increase when the sensor forms either soluble oligomers or insoluble aggregates. Herein, we present protocols that describe how the AgHalo sensor can be employed to visualize and quantify proteome stress in live cells using a direct ?uorescence read-out and visualization with a ?uorescence microplate reader and a microscope. Additionally, protocols for using the AgHalo sensor in combination with ?uorogenic probes and commercially available HaloTag probes to enable two-color imaging experiments are described. These protocols will enable use of the AgHalo sensor to visualize and quantify proteostasis in live cells, a task that is dif?cult to accomplish using previous, always-?uorescent methods.