研究目的
To develop NIRS calibrations using diverse Napier grass germplasm grown over 3 years to predict fiber components and conversion efficiency to ethanol via biochemical techniques to rapidly screen plant material for genetic improvement.
研究成果
The developed NIRS calibrations accurately estimate compositional characteristics and ethanol yield in Napier grass, enabling rapid and inexpensive screening for genetic improvement. This will aid in the development of Napier grass cultivars with improved ethanol yields.
研究不足
The study focused on a specific set of Napier grass accessions and may not represent all genetic diversity. The NIRS calibration for xylose was not as strong as for other components.
1：Experimental Design and Method Selection:
The study involved selecting thirty genetically diverse Napier grass accessions for analysis. Near-infrared reflectance spectroscopy (NIRS) was used to develop calibrations for predicting ethanol production, xylans, nitrogen concentration, and ash by correlating with wet chemistry analyses.
2：Sample Selection and Data Sources:
Samples were harvested from a germplasm nursery in Tifton, GA, over three years (2005-2007). Leaves and stems were separated, dried, ground, and sieved for analysis.
3：7). Leaves and stems were separated, dried, ground, and sieved for analysis. List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment included a near-infrared spectrometer (Foss 6500), Ankom filter bag method for fiber determination, and HPLC for measuring glucose, xylose, and ethanol.
4：Experimental Procedures and Operational Workflow:
Samples were pretreated with dilute sulfuric acid, followed by simultaneous saccharification and fermentation (SSF) to measure ethanol and xylose yields. NIRS calibrations were developed and validated.
5：Data Analysis Methods:
Statistical analysis was performed using SAS v. 9.2, including GLM procedure and Pearson correlation coefficients.
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