研究目的
Developing an analytical method of AA with simplicity, reliability and high selectivity for analytical applications and clinical disease diagnosis.
研究成果
The developed dual-ratiometric fluorescence and colorimetric dual-readout method for highly selective AA detection based on NRR performance of DHAA and the excellent optical property of DFQ and OPDox is successful. The sensing assay with fluorescent and colorimetric dual modes could output a variety of signals and make the analytical results more convincing. The sensor also can be used for the detection of AA in real serum samples with satisfactory results and good repeatability.
研究不足
The method may be affected by high concentrations of Cl- which can produce insoluble salts with Ag+, interfering with the detection of target. However, this can be mitigated by using Hg2+ to form a more insoluble precipitated salt with Cl-.
1：Experimental Design and Method Selection:
The NRR strategy involves three processes: Ag+ oxidated OPD to OPDox, AA inhibited the generation of OPDox and the AA was oxidized to DHAA. The specific performance of DHAA NRR, due to the condensation reaction between dicarbonyl group of DHAA and diamine group of OPD, was confirmed by ESI-MS and 1H NMR spectra analysis.
2：Sample Selection and Data Sources:
Human serum samples were treated by acetonitrile to remove impurities before using in the experiment.
3：List of Experimental Equipment and Materials:
UV-2450 spectrophotometer, F-7000 fluorescence spectrophotometer, Bruker AVB-500 spectrometer, ZQ2000 mass spectrometer.
4：Experimental Procedures and Operational Workflow:
Different concentrations of AA, 200 μM AgNO3 and
5：5 mM OPD were mixed in the 37 oC for 30 min under the Tris-HAc (10 mM, pH = 7) buffer. Then the fluorescence and colorimetric spectra of the above incubated solution were obtained at room temperature (25 ± 0 oC). Data Analysis Methods:
The ultraviolet intensity ratio and emission intensity ratio change were recorded after adding different concentrations of AA by UV–vis spectra and fluorescence spectra.
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