研究目的
To clarify the effects of diamond-like carbon (DLC) thin film and the wrinkle microstructure formed by depositing the DLC thin film on a poly(dimethylsiloxane) (PDMS) substrate on cell proliferation with a view to applying to cell scaffolds.
研究成果
The DLC thin film deposited PDMS substrates are expected to be a potential candidate as cell scaffolds due to their ability to enhance cell proliferation and spreading. The microstructure formed by DLC thin film deposition had the optimum range of the height difference of the wrinkle for the cell growth.
研究不足
The method to form a wrinkle structure by DLC deposition had a limitation in creating larger roughness, and substrates having Rc of more than several μm were not able to be obtained.
1：Experimental Design and Method Selection:
The study used an inductively-coupled plasma (ICP) system for DLC thin film deposition on PDMS and silicon substrates.
2：Sample Selection and Data Sources:
Mouse myoblast cells (C2C12) were cultured on substrates with/without DLC thin film and wrinkle microstructure.
3：List of Experimental Equipment and Materials:
ICP system, PDMS substrates, silicon wafers, DLC thin films.
4：Experimental Procedures and Operational Workflow:
Substrates were prepared with varying surface roughness by adjusting argon plasma irradiation and DLC deposition times. Cell culture tests were conducted, and cell proliferation was analyzed.
5：Data Analysis Methods:
Cell nuclei and F-actins were stained and observed using a fluorescence microscope. Image analysis was performed using ImageJ software.
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