研究目的
Investigating the effects of X-ray irradiation on nuclear and membrane regions of single SH-SY5Y human neuroblastoma cells using Raman micro-spectroscopy to identify biochemical changes and potential markers of apoptosis.
研究成果
Raman micro-spectroscopy combined with i-PCA analysis identified biochemical changes in single neuroblastoma cells following X-ray irradiation, particularly in the nucleus region. These changes were associated with cell-cycle perturbation and potential markers of apoptosis. Repair processes were observed 24 h post-irradiation, indicating the cells' ability to recover from X-ray-induced damage.
研究不足
The study was conducted in vitro, and the findings may not fully represent the in vivo scenario. The repair processes observed 24 h post-irradiation suggest that some damage may be transient, limiting the detection of long-term effects.
1：Experimental Design and Method Selection:
Raman micro-spectroscopy was used to investigate biochemical changes in single SH-SY5Y human neuroblastoma cells after X-ray irradiation. The spectra were analyzed using PCA and i-PCA methods.
2：Sample Selection and Data Sources:
SH-SY5Y human neuroblastoma cells were cultured in vitro and irradiated with graded X-ray doses (2, 4, 6, 8 Gy).
3：List of Experimental Equipment and Materials:
A commercial Raman micro-spectrometer (LabRam, Jobin-Yvon Horiba) equipped with an external Ar+ laser was used for measurements.
4：Experimental Procedures and Operational Workflow:
Cells were fixed immediately after irradiation and 24 h post-irradiation. Raman spectra were recorded from the nucleus and membrane regions of single cells.
5：Data Analysis Methods:
The spectra were analyzed using PCA and i-PCA to identify biochemical changes induced by X-ray irradiation.
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