研究目的
To describe a fully automated integrated system for forensic short tandem repeat (STR) analysis of reference samples, achieving buccal swab-in and DNA pro?le-out.
研究成果
The MiDAS system employs a self-contained micro?uidic cartridge with four modular components: buccal swab lysis and DNA extraction, multiplexed PCR, PCR products transfer for analysis, and microchip CE separation. It is a flexible and versatile platform that can be adapted to different assays.
研究不足
The technical and application constraints of the experiments, as well as potential areas for optimization, are not explicitly mentioned in the provided text.
The methodology involves the integration of several operational elements onto a single micro?uidic platform, including substrate lysis, lysate DNA extraction, single or multiplexed PCR ampli?cation, amplicon separation, and product readout. The system is designed for forensic short tandem repeat (STR) analysis of reference samples.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容-
UV Epoxy
Dymax 1180-MT
Dymax
Used for gluing electrodes in place
-
Silastic tubing
Cat. No. 508-006
Dow Corning
Used for transfer to CE
-
Microscope objective
Olympus LUCPLFLN
Olympus
Used in optical setup
-
Thermally activated single-shot polymer valves
Sigma-Aldrich#411663-1Kg
Sigma-Aldrich
Used for opening and closing valves in the cartridge
-
polycarbonate sheet
Bayer Makrolon
Bayer
Used as substrate and capping layer in cartridge fabrication
-
Pressure-sensitive adhesive
ARcare 90106
Adhesives Research
Used as a two-sided hybrid acrylic adhesive system in cartridge fabrication
-
Al wire
Alfa Aesar 43427-G5
Alfa Aesar
Used as electrodes for the electrochemical pumps
-
PEEK tubing
IDEX 1568
IDEX
Used for CE transfer
-
Ethanol
Cat. No. 2701
Fisher Scientific
Used to clean the cartridge
-
ChargeSwitch gDNA Normalized Buccal Cell Kit
CS11020
Life Technologies
Used for buccal gDNA extraction
-
Buccal swab
WB100035
Whatman Omni Swab
Used for sample collection
-
PowerPlex ESI16 system
Promega
Used for multiplex amplification
-
Hi-Di Formamide solution
Life Technologies
Used in denaturation solution
-
WEN Internal Lane Standard
ILS500
Promega
Used as size standard
-
Laser source
Cobolt Calypso
Used for detection
-
CCD sensor
Sony S7031
Sony
Used as detector
-
Microchip device
Micronit Microfluidics B.V.
Used for CE separation
-
Separation matrix
Sigma-Aldrich
Used in CE separation
-
登录查看剩余16件设备及参数对照表
查看全部
