研究目的
To separate anti-LPS antibodies from human serum using E. coli strain BL21(DE3) and its modified ClearColi? strain, and to demonstrate their applicability in detecting gram-negative bacteria through fluorescence and electrochemical assays.
研究成果
The separated anti-LPS antibodies showed selective binding to LPS-carrying bacteria and were effectively used in fluorescence and electrochemical assays for detecting gram-negative bacteria. The method presents a potential approach for the detection of bacterial infections.
研究不足
The study is limited to the in vitro detection of gram-negative bacteria using the separated anti-LPS antibodies. The applicability in clinical or environmental samples was not explored.
1：Experimental Design and Method Selection:
The study involved the separation of anti-LPS antibodies from human serum using E. coli BL21(DE3) and ClearColi?, followed by their characterization and application in immunoassays.
2：Sample Selection and Data Sources:
Human serum was used as the source of anti-LPS antibodies. E. coli strains BL21(DE3) and ClearColi? were cultured for the experiments.
3：List of Experimental Equipment and Materials:
Included FACSCalibur analyzer, Monolith NT.115 instrument, Victor X5 plate reader, and various chemicals and reagents.
4：Experimental Procedures and Operational Workflow:
The separation process involved binding serum proteins to BL21(DE3), dissociation with acid, filtering with ClearColi, and separation by protein-A column chromatography.
5：Data Analysis Methods:
Flow cytometry and thermophoretic immunoassay were used for analysis.
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