研究目的
To prepare hydrophilic and homogeneous sub-10 nm blue light-emitting gold nanoparticles (NPs) functionalized with different capping agents and study their structure, average size, surface characteristics, stability at different pH values, photoluminescence, and cytotoxicity for biomedical applications.
研究成果
Highly soluble and stable sub-10 nm Au NPs functionalized with different capping agents were prepared. Citrate capped gold NPs showed high size monodispersion with modifications. Thiol capped gold NPs showed homogeneous size distribution with particularly small average size for GSH capped NPs. All NPs showed high biocompatibility with low cytotoxicity even at high concentration, with Au-GSH NPs showing toxicity and inhibiting proliferation of tumor cells, opening avenues for additional applications.
研究不足
The study focuses on sub-10 nm gold nanoparticles and their biomedical applications, but the cytotoxicity and photoluminescence properties may vary with different sizes and capping agents not studied here.
1：Experimental Design and Method Selection:
Four different types of gold NPs were synthesized starting from the reduction of Au3+ cations present in the HAuCl
2：Two different reducing agents were used:
sodium citrate for synthesis A and B, and sodium borohydride for synthesis C and D.
3：Sample Selection and Data Sources:
Samples were prepared by depositing 10 μL of an Au NP colloidal solution that was drop-casted onto a holey-carbon-coated Cu grid and dried for 5 h.
4：List of Experimental Equipment and Materials:
Transmission electron microscopy (FEI Nova NanoSEM 450, JEOL 2010), dynamic light scattering (Nanotrac Wave), Fourier transformed infrared spectrophotometry (Bruker Tensor 37), UV-Vis spectrophotometer (Lambda 19 PerkinElmer), fluorometer (PTI Quantamaster), LaserStrobe technique by using a nitrogen/dye laser GL-
5：Experimental Procedures and Operational Workflow:
33 The synthesis involved heating HAuCl4 aqueous solution and adding sodium citrate or sodium borohydride as reducing agents, followed by stirring and cooling.
6：Data Analysis Methods:
Size distribution obtained from TEM micrographs, UV-Vis absorption spectra for LSPR band analysis, PL emission spectra for photoluminescence behavior, and cytotoxicity assays.
独家科研数据包，助您复现前沿成果，加速创新突破
获取完整内容
