研究目的
Investigating the spatial and temporal resolutions of bio-imaging with magnetic nanoparticles (MNP) as a label and a diamond substrate as a magnetic field imager.
研究成果
The study demonstrates the feasibility of detecting 1-μm MNPs on a diamond substrate with high spatial and temporal resolution using NV centers. It suggests that incorporating already reported sensitivity enhancement technologies could achieve a spatiotemporal resolution of <1 s and <1 μm, despite the current limitations.
研究不足
The study notes that for measurement in culture medium, an objective lens with longer working distance is required, which could worsen the condition. Additionally, the theoretical limit of temporal sensitivity is more than four orders of magnitude smaller than measured, indicating room for improvement in sensitivity.
1：Experimental Design and Method Selection:
The study utilized a diamond substrate with a high-density, perfectly oriented NV centers in a CVD film for magnetic field imaging of superparamagnetic particles. The method involved measuring relative fluorescence at four operation points corresponding to the steepest slopes of two dips in the ODMR spectrum.
2：Sample Selection and Data Sources:
A type-Ib (111) diamond substrate with a 3.5 μm CVD layer was used. The MNPs used were superparamagnetic with 1-μm diameter.
3：5 μm CVD layer was used. The MNPs used were superparamagnetic with 1-μm diameter.
List of Experimental Equipment and Materials:
3. List of Experimental Equipment and Materials: Equipment included an objective lens (Olympus UPLSAPO 60XS2 oil immersion, NA 1.30), an image sensor (Hamamatsu photonics C11440-22CU), and a microwave coil on a printed circuit board.
4：30), an image sensor (Hamamatsu photonics C11440-22CU), and a microwave coil on a printed circuit board.
Experimental Procedures and Operational Workflow:
4. Experimental Procedures and Operational Workflow: The setup involved drying MNPs on a diamond substrate and detecting the fluorescence image of the NV centers through an objective lens. The ODMR spectrum was measured to detect the magnetic field, with microwave frequencies locked to operation points at the steepest slopes of dips in the ODMR spectrum.
5：Data Analysis Methods:
The magnetic field was obtained by analyzing the ODMR spectrum detected at every pixel on the image-sensor, leading to mapping of MNPs.
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