研究目的
To develop a noninvasive technique for the detection of vulnerable plaques using scatter analyses with flow cytometry (FCM) method combined with the diffusion reflection (DR) method.
研究成果
The combination of FCM and DR measurements provides a potential novel, highly sensitive, and noninvasive method for the identification of atherosclerotic vulnerable plaques. This work paves the way toward a novel clinical method for the in vitro and, in the following, in vivo detection of AS.
研究不足
The study is a preliminary investigation and further research is needed to understand the dependence between the GNP’s shape and size and their uptake by different kinds of MΦ. Additionally, more specific FCM analyses to identify different M subtypes following their incubation with different GNP should be performed.
1：Experimental Design and Method Selection:
The study utilized gold nanorods (GNR) for their unique optical properties to detect macrophages (MΦ) uptake in atherosclerotic plaques. The FCM method was used based on their scattering properties, and the DR method was used based on their unique absorption properties.
2：Sample Selection and Data Sources:
Macrophages were prepared and loaded with GNR. The GNR uptake by MΦ was analyzed by FCM scatter measurements.
3：List of Experimental Equipment and Materials:
FACSAria III cell sorter (BD Biosciences), spectrophotometer (Shimadzu Corporation), transmission electron microscopy, laser diode with wavelengths of 650 nm and 780 nm, optical fiber (Linear Photonics LLC), digital scope (Mso7034a; Agilent Technologies), LabView program (National Instruments).
4：Experimental Procedures and Operational Workflow:
MΦ were incubated with GNR, and the uptake was analyzed by FCM. DR measurements were performed on tissue-like phantoms containing MΦ and GNR.
5：Data Analysis Methods:
FCM data were analyzed with the FACSDiva software. DR data were processed using the LabView program.
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