研究目的
To investigate the thermal diffusivity of thermally treated M13 phage-based assemblies (films) and demonstrate the maintenance of high thermal diffusivity in smectic liquid crystalline-oriented M13 phage-based assemblies after high temperature treatment.
研究成果
The high thermal diffusivity of smectic liquid crystalline oriented assemblies of M13 phages remained unchanged by thermal treatment, demonstrating structural stability. This suggests the potential for using biomolecular assemblies as reliable thermal conductive materials.
研究不足
The study focuses on the thermal stability of M13 phage-based assemblies at 150 ?C, which may not cover all potential high-temperature applications. The structural changes observed in nematic liquid crystalline oriented assemblies suggest limitations in their thermal stability.
1：Experimental Design and Method Selection
The study involved preparing M13 phage-based films with liquid crystalline orientation, thermally treating them at 150 ?C for 30 min, and then characterizing their thermal diffusivity and structural stability using various microscopic and spectroscopic techniques.
2：Sample Selection and Data Sources
M13 phages were expressed using the Ph.D. Peptide Display Cloning System, amplified, and purified. Films were prepared by mounting M13 phage solutions on glass plates patterned with a highly water-shedding coating.
3：List of Experimental Equipment and Materials
Polarized optical microscope (Eclipse LV100ND, Nikon), AFM (SPM-9600, Shimadzu), thermal diffusivity measurement device (ai-Phase mobile 1u, ai-Phase Co. Ltd.), SAXS equipment (BL-10C and 6A of Photon Factory in KEK).
4：Experimental Procedures and Operational Workflow
Films were observed under POM and AFM before and after thermal treatment. Thermal diffusivity was measured using temperature wave analysis. SAXS measurements were performed to examine the structural stability.
5：Data Analysis Methods
Thermal diffusivity was calculated from the relationship of the square root of the angular frequency and the phase delay of the temperature wave. SAXS data were analyzed using homemade GUI software to determine the degree of orientation.
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