研究目的
To develop a visualized quantitative detection method for nucleic-acid amplification tests based on the coffee-ring effect on a colloid-crystal substrate.
研究成果
The developed method enables rapid, sensitive, and quantitative detection of trace DNA using the coffee-ring effect on a colloid-crystal substrate, suitable for point-of-care testing under resource-limited conditions.
研究不足
The method's sensitivity and reproducibility may be influenced by environmental humidity and the uniformity of the colloid-crystal substrate.
1：Experimental Design and Method Selection:
The study utilizes the coffee-ring effect on a colloid-crystal substrate for the quantitative detection of nucleic acids. The method involves dropcasting a solution for loop-mediated isothermal amplification (LAMP) of DNA on the substrate and observing the formation of a coffee ring after drying.
2：Sample Selection and Data Sources:
The study uses DNA samples from Salmonella, diluted to various concentrations, to demonstrate the applicability of the method.
3：List of Experimental Equipment and Materials:
Includes a colloid-crystal substrate made of self-assembled monodisperse SiO2 nanoparticles, a homemade dry chamber with constant humidity, and a smartphone for quantitative measurements.
4：Experimental Procedures and Operational Workflow:
The LAMP reaction mixture is incubated at 65 °C for 60 min. The amplified products are then dropcast onto the colloid-crystal substrate and allowed to dry in the chamber for 5 min, forming a coffee ring.
5：Data Analysis Methods:
The width of the coffee ring is measured and correlated to the original DNA concentration using a smartphone app for quantitative detection.
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