研究目的
Investigating the feasibility and performance of a K-domain transform based three-dimensional microscopy technique for high-speed and high-resolution imaging of specimens.
研究成果
The proposed K-domain transform based 3D imaging method is feasible and capable of achieving high-speed and high-resolution imaging. It offers a simple optical setup that can be realized with slight modifications to a common commercial microscope.
研究不足
The axial resolution is limited by the detector's sensitivity, and the technique requires samples to be roughly transparent to avoid crosstalk between different layers.
1：Experimental Design and Method Selection:
The technique involves illuminating an object with a light sheet and recording the complex amplitude of the reflected light. The structure of the illuminated slice is reconstructed by transforming the reflected light from the vertical plane to the axial plane using a K-domain transform.
2：Sample Selection and Data Sources:
Biological samples and glass samples with fabricated structures were used to verify the technique's feasibility and performance.
3：List of Experimental Equipment and Materials:
A CW He-Ne laser, cylindrical lens, CCD camera (AVTFB421), and samples including a piece of glass and a damaged optical filter.
4：Experimental Procedures and Operational Workflow:
The sample is illuminated with a light sheet, and the reflected light is recorded as an offline hologram. The complex amplitude is then transformed to reconstruct the sample's structure.
5：Data Analysis Methods:
The Fourier transform and inverse Fourier transform are used to analyze the recorded light field and reconstruct the sample's image.
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