研究目的
To present recent developments in X-ray tomography using in-line phase contrast and their applications to mineralised tissue, including bone and artificial biomaterials, at micro- and nanoscale.
研究成果
The chapter highlights the potential of in-line X-ray phase contrast tomography in regenerative medicine, showcasing its applications in nano-tomography of bone and micro-tomography of bone formation. Despite its current limited use, the examples provided aim to stimulate further research in this field.
研究不足
The technique is still relatively little used in the field of regenerative medicine, and there are challenges in imaging heterogeneous objects, especially if quantitative results are required. The method also requires careful calibration and cannot be used in in situ and high frame rate imaging due to the requirement of images at several distances.
1：Experimental Design and Method Selection:
The chapter discusses the use of in-line X-ray phase contrast imaging for studying mineralised tissue, focusing on the advancements in reconstruction algorithms to improve resolution and quantitative imaging.
2：Sample Selection and Data Sources:
Samples include bone and artificial biomaterials, with applications in nano-tomography of bone ultrastructure and micro-tomography of bone formation in artificial bone grafts and healthy growing mice.
3：List of Experimental Equipment and Materials:
Utilizes X-ray optics for pushing resolution towards the nanoscale and advanced priors in reconstruction for quantitative imaging.
4：Experimental Procedures and Operational Workflow:
Describes the setup for in-line X-ray phase contrast μCT and nano-CT, including the use of X-ray reflective optics for geometric magnification in nano-CT.
5：Data Analysis Methods:
Discusses phase retrieval algorithms and the use of attenuation tomograms as prior knowledge for reconstructing the phase shift in the object.
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